An enrichment method for temperature-sensitive and auxotrophic mutants of yeast
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چکیده
منابع مشابه
An enrichment for temperature-sensitive mutants in Tetrahymena thermophila.
The parameters for the killing of Tetrahymena by 5-bromodeoxyuridine(BUdR) and near-ultraviolet light have been determined. Significant preferential killing by UV of cells that have incorporated BUdR was obtained when the cells were irradiated in a nonnutrient buffer. UV alone was found to be toxic to cells irradiated in growth medium. Mutants defective in division at a restrictive temperature ...
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Three mutants of the yeast Saccharomyces cerevisiae which require exogenous ethanolamine or choline were isolated. The mutants map to a single locus (cho1) on chromosome V. The lipid composition suggests that cho1 mutants do not synthesize phosphatidylserine under any growth conditions. If phosphatidylethanolamine or phosphatidylcholine, which are usually derived from phosphatidylserine, were s...
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A system is described for isolating temperature-sensitive mutants of Saccharomyces cerevisiae with defects in early meiotic events. We used an otherwise haploid strain disomic (n+1) for chromosome III, and heteroallelic at the leucine-2 locus. Meiotic development was initiated by exposure of the strain to acetate sporulation medium, and monitored by the appearance of leucine-independent intrage...
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The consequences of two different mutations in the single essential actin structural gene of yeast (Saccharomyces cerevisiae) were studied. Both conditional-lethal actin mutants exhibit six phenotypes at the restrictive temperature: disruption of the asymmetric staining pattern of actin assembly; delocalized deposition of chitin on the cell surface; partial inhibition of secretion of the peripl...
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In order to investigate the mechanisms of macromolecule synthesis and their regulation in a eucaryotic organism, we have undertaken an investigation of temperature-sensitive mutants in yeast. The isolation of temperature-sensitive mutants of Saccharomyces cerevisiae and their characterization with respect to macromolecule synthesis, morphology, and cell division at the nonpermissive temperature...
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ژورنال
عنوان ژورنال: Molecular and General Genetics MGG
سال: 1979
ISSN: 0026-8925,1432-1874
DOI: 10.1007/bf00274022